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KMID : 0616619950010010391
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Journal of Soonchunhyang Medical College
1995 Volume.1 No. 1 p.391 ~ p.400
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The Effect of Sodium-Citrate on the Multiplication of Vibrio vulnificus
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Abstract
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The efficiency of selective isolation of V. vulnificus with current various agar plate media seemed to be significantly poor. So far, TCBS agar has been most widely used for Vibrio species. The fact that several ingredients of TCBS agar inhibit growth of other contaminated organisms and ultimately enhance selective growth of Vibrio species could also inhibit Vibrio vulni{ccus should not be oversighted. Moreover differentiation between V. vulnificus and V. parahaemolyticus by morphology of colony grown on TCBS found to be quietly difficult.
On these regards, the effect of each ingredient of TCBS agar including sodium citrate on the growth of V. vulnificus was needed to examine carefully in order to develop a better selective medium for V. vulnfflcus. A good many characteristics could be found through this experiment and these data could be applied for developing new medium.
1. V. vulni7cus once transformed from opaque colony to translucent colony by given growth condition (in broth culture) was never changed
2. The growth of V. vulnificus appeared to be inhibited on agar plate medium by addition of low concentration of sodium citrate. The more concentration of sodium citrate was in medium, the poorer growth of V. vulnificus was observed.
3. Ferric ammonium citrate alone did not affect the growth of V. vulnificus.
4. Ferric nitrate and sodium thiosulfate markedly inhibited the growth of V. vulnificus. Conclusively, for the purpose of the growth of V. vulnificus there should be some modification of ingredients of TCBS agar or a newly designed medium other than TCBS agar, especially in case of V. vulnificus.
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